Characteristics of Traveler with Middle East Respiratory Syndrome, China, 2015

نویسندگان

  • Wen Da Guan
  • Chris Ka Pun Mok
  • Zi Lin Chen
  • Li Qiang Feng
  • Zheng Tu Li
  • Ji Cheng Huang
  • Chang Wen Ke
  • Xilong Deng
  • Yun Ling
  • Shi Guan Wu
  • Xue Feng Niu
  • Ranawaka A Perera
  • Yuan Da Xu
  • Jincun Zhao
  • Lin Qi Zhang
  • Yi Min Li
  • Rong Chang Chen
  • Malik Peiris
  • Ling Chen
  • Nan Shan Zhong
چکیده

Throat swab, nasal swab, sputum, urine, serum, fecal, and environmental samples were collected from the patient and placed in viral transport medium. Viral RNA was extracted from samples by using the QIAamp MinElute Virus Spin Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. Middle East respiratory syndrome coronavirus (MERS-CoV) was detected by using real-time reverse transcription PCR with primers upE-Fwd (5-GCAACGCGCGATTCAGTT3) and upE-Rev (5-GCCTCTACACGGGACCCATA-3), and probe upE-Prb (6carboxyfluorescein[FAM]-5-CTCTTCACATAATCGCCCCGAGCTCG-3-6carboxy-N,N,N,N-tetramethylrhodamine [TAMRA]).

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عنوان ژورنال:

دوره 21  شماره 

صفحات  -

تاریخ انتشار 2015